MAB13431
Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2
clone A-Gel VC2, Chemicon®, from mouse
别名:
Gelatinase A, 72 kDa Type IV Collagenase
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A-Gel VC2, monoclonal
Application:
ELISA
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Species reactivity:
human
Citations:
7
Technique(s):
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
Uniprot accession no.:
产品名称
Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2, clone A-Gel VC2, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
A-Gel VC2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MMP2(4313)
Analysis Note
Control
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
Application
Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).
Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Biochem/physiol Actions
MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.
CELLULAR LOCALIZATION:
Cytoplasmic.
CELLULAR LOCALIZATION:
Cytoplasmic.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.
Immunogen
Epitope: pro and active form
Human APMA-activated native 72kDa Gelatinase A.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1048
Physical form
10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide
Format: Purified
Preparation Note
Antibody is stable for 12 months when stored at 2-8ºC
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells.
Marano, G; Gronewold, C; Frank, M; Merling, A; Kliem, C; Sauer, S; Wiessler, M; Frei et al.
Beilstein Journal of Organic Chemistry null
A B Frey et al.
Histopathology, 50(6), 720-726 (2007-05-12)
Osteopontin (OPN) is a matricellular protein involved in tissue remodelling, cell-mediated immunity and malignant transformation. High OPN serum levels predict poor prognosis in non-small cell carcinoma and set patients with malignant pleural mesothelioma (MM) apart from disease-free asbestos-exposed individuals. Yet
Moa Lindgren et al.
Cancers, 14(14) (2022-07-28)
Circulating type IV collagen (cCOL IV) is a potential biomarker for patients with colorectal liver metastases (CLM) who present with elevated levels of COL IV in both CLM tissue and circulation. This study aimed to establish the cellular origin of
Bartlomiej Perek et al.
BioMed research international, 2013, 730721-730721 (2013-10-24)
Migration of the smooth muscle cells (SMCs) to the tunica media in the saphenous vein (SV) transplants is facilitated by matrix metalloproteinases (MMPs). The aim of this study was to identify any associations between expression of MMP-2 or endogenous tissue
Bartlomiej Perek et al.
Folia histochemica et cytobiologica, 54(2), 91-98 (2016-11-16)
Currently, elderly people constitute a large proportion of patients undergoing coronary artery bypass grafting (CABG). Activated smooth muscle cells in the tunica media of saphenous vein (SV) grafts are thought to play a key role in the formation of neointima
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