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MAB1281

Sigma-Aldrich

抗核抗体,克隆235-1

clone 235-1, Chemicon®, from mouse

别名:

HuNu

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

235-1, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

输入

sample type neural stem cell(s)

同位素/亚型

IgG1

运输

dry ice

靶向翻译后修饰

unmodified

一般描述

干细胞或胚胎细胞疗法等细胞替代技术对许多疾病具有巨大的应用潜力。 人与动物细胞或组织之间的跨物种移植需要在宿主环境中鉴定和定位供体材料。抗核抗体是有用的细胞学和组织学标志物,可用于鉴定异种移植模型中的人类细胞。

特异性

人核单克隆抗体与人细胞特异性反应。 在免疫组化中,它对小鼠、大鼠、鸡没有反应性。 尚未测试其他哺乳动物或动物物种的反应性。
染色所有人类细胞类型的细胞核,使细胞核呈弥漫性染色。 中期细胞的染色体染色呈阴性。还可以染色灵长类动物的细胞核(Sanchez-Pernaute, 2005)。抗体不与小鼠或大鼠的细胞核反应。

应用

抗核抗体,克隆235-1已用于:免疫组织化学(1:200);人细胞核免疫标记(HuNu) (1:300);颌下腺的组织学和免疫荧光分析

外形

形式:纯化
杂交瘤上清液。溶于PBS中的液体。不含防腐剂。
纯化的蛋白 A

储存及稳定性

自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。

分析说明

对照
所有人类细胞类型

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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Human stem cell delivery for treatment of large segmental bone defects.
Dupont, KM; Sharma, K; Stevens, HY; Boerckel, JD; Garcia, AJ; Guldberg, RE
Proceedings of the National Academy of Sciences of the USA null
E Josue Ruiz et al.
Nature communications, 5, 3175-3175 (2014-01-17)
The signals that regulate stem cell self-renewal and differentiation in the lung remain elusive. Lung stem cells undergo self-renewal or lineage commitment to replenish tissue, depending on cross-talk with their environment. This environment, also known as the niche, includes mesenchymal
Yuelin Zhang et al.
Cell transplantation, 21(10), 2225-2239 (2012-07-11)
Although transplantation of adult bone marrow mesenchymal stem cells (BM-MSCs) holds promise in the treatment for pulmonary arterial hypertension (PAH), the poor survival and differentiation potential of adult BM-MSCs have limited their therapeutic efficiency. Here, we compared the therapeutic efficacy
Jun Lei et al.
American journal of obstetrics and gynecology, 212(5), 639-639 (2015-01-04)
Using a mouse model of intrauterine inflammation, we have demonstrated that exposure to inflammation induces preterm birth and perinatal brain injury. Mesenchymal stem cells (MSCs) have been shown to exhibit immunomodulatory effects in many inflammatory conditions. We hypothesized that treatment
Ji Hyun Kim et al.
Scientific reports, 6, 38754-38754 (2016-12-08)
Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however

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探索抗体处理基础知识,包括有关结构、类别和正常免疫球蛋白系列的技术信息。

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

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实验方案

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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