Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9

Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism.

Epitope: alpha subunit, azide free

IM-9 lymphocytes followed by purified insulin receptor.

Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 detects level of Insulin Receptor & has been published & validated.

Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM

Antagonist: Blocks insulin action

Does not work in immunoprecipitation {Soos, 1986}.

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Protein G Purified

Format: Purified

Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
3T3 cells expressing transfected human insulin receptor

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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