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Merck
CN
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IPFL00010

Millipore

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

别名:

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC代码:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-FL PVDF膜, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

物料

PVDF membrane
plain filter
white filter

质量水平

400

特点

hydrophobic

制造商/商品名称

Immobilon®

技术

dot blot: suitable
western blot: suitable

过滤器长度 × 宽度

27 cm × 3.75 m

孔径

0.45 μm pore size

容量

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

检测方法

chemiluminescent
colorimetric
fluorometric

运输

ambient

相关类别

一般描述

Immobilon®-FL膜是一种聚偏氟乙烯(PVDF)微孔膜，标称孔径为0.45 μm。它被设计用来结合从各种凝胶基质转移的蛋白质。Immobilon®-FL转移膜是疏水的，采用均匀的孔隙结构，具有高生物分子结合能力。该膜为分子量大于20,000的蛋白质提供了最佳印迹。Immobilon®-FL膜在很宽的激发/发射波长范围内显示非常低的自荧光。这种特性使其成为包括荧光免疫检测在内的印迹应用的理想材料。Immobilon®-FL膜也可用于标准化学发光或显色检测。

应用

Immobilon-FL PVDF膜已用于蛋白质印迹分析。

特点和优势

Immobilon-FL膜：
  • 首款为荧光应用优化的转移膜；极低的背景提高了所有荧光检测协议的灵敏度
  • 在广泛的激发/发射波长范围内兼容所有常用的荧光探针
  • 最适合多路复用和化学荧光检测
  • 兼容标准阻断剂和缓冲液

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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S A Misek et al.
Oncogene, 39(7), 1466-1483 (2019-10-30)
Over half of cutaneous melanoma tumors have BRAFV600E/K mutations. Acquired resistance to BRAF inhibitors (BRAFi) remains a major hurdle in attaining durable therapeutic responses. In this study we demonstrate that ~50-60% of melanoma cell lines with vemurafenib resistance acquired in
Guangchuan Wang et al.
Biological research, 52(1), 60-60 (2019-12-19)
Recent studies have confirmed that RASAL1 has an antitumor effect in many cancers, but its functional role and the molecular mechanism underlying in colon cancer has not been investigated. We collected human colon cancer tissues and adjacent non-tumor tissues, human
David J Ferland et al.
PloS one, 15(2), e0229251-e0229251 (2020-02-25)
Since chemerin's identification as an adipokine, it has been associated with a number of human diseases including diabetes and obesity. However, the basic scientific foundation for these clinical determinations is still lacking. Fibroblastic mouse 3T3 cells are unable to develop
Enrique Calvo et al.
Science advances, 6(26), eaba7509-eaba7509 (2020-07-09)
Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here
Daniel Del Valle Morales et al.
Nucleic acids research, 48(7), 3806-3815 (2020-01-31)
Cap homeostasis is the cyclical process of decapping and recapping that maintains the translation and stability of a subset of the transcriptome. Previous work showed levels of some recapping targets decline following transient expression of an inactive form of RNMT
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