Milli-Mark^® Anti-Integrin αVβ3 Antibody, clone LM609-FITC

The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.

Antibody recognizes Integrin αVβ3.

Purified adhesion receptor from the human melanoma cell line M21 (Cherish, D. and Spiro, R. (1987) J. Biol. Chemistry, 262(36):17703-17711).

Detect Integrin αVβ3 using this Milli-Mark Anti-Integrin αVβ3 Antibody, clone LM609-FITC validated for use in FC.

Research Category
Cell Structure

Research Sub Category
Integrins

Evaluated by flow cytometry using U937 cells.

Protein A purified

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Flow Cytometry
Antibody dilution for cellular staining:

• Prepare an antibody working solution by diluting the primary antibody 1:5 with PBS
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Control
U937 cells

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