产品名称
Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6), liquid, clone DCS-6, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
DCS-6, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoblotting (1 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation
Neutralization Studies
Paraffin Sections (5 µg/mL, heat pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)
Analysis Note
Negative Control
trp E (Ab-1)
trp E (Ab-1)
Positive Control
MCF7, or A431 cells or breast carcinoma tissue
MCF7, or A431 cells or breast carcinoma tissue
Application
Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).
Immunogen
Human
recombinant human cyclin D1
Other Notes
Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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