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一般描述
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm′s tumor protein.
Recognizes the ~47-55 kDa Wilm′s tumor protein in Wilm′s tumor tissue.
This Anti-Wilm′s Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm′s Tumor Protein.
免疫原
Human
a synthetic peptide corresponding to amino acids from the C-terminus of human Wilm′s tumor protein
应用
Immunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 10 mM PBS, 0.2% BSA, pH 7.6.
重悬
Following initial use, aliquot and freeze (-20°C) for long-term storage.
分析说明
Positive Control
Wilm′s tumor tissue
Wilm′s tumor tissue
其他说明
Huff, V. 1998. Am. J. Med. Genet.79, 260.
Staining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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