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一般描述
IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
特异性
Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD.
The antibody reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.
免疫原
Epitope: Human IgM, IgG, IgA
Prepared from pepsin digest of goat anti-human Ig.
应用
Detect Human IgM+IgG+IgA using this Goat anti-Human IgM+IgG+IgA Antibody, F(ab′)2, PE conjugate validated for use in ELISA & IF.
Immunofluorescence: ≤0.1 μg/10sup6 cells.
ELISA:
Optimal working dilutions must be determined by the end user.
ELISA:
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
外形
Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN3.
储存及稳定性
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Do not freeze.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
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