推荐产品
生物来源
goat
质量水平
偶联物
biotin conjugate
抗体形式
F(ab′)2 fragment of affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
种属反应性
rabbit
制造商/商品名称
Chemicon®
浓度
1.1 mg/mL
技术
ELISA: suitable
western blot: suitable
运输
wet ice
靶向翻译后修饰
unmodified
特异性
Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)
应用
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications:
1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000-1:100,000 for western blotting with chemiluminescent substrates.
1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.
1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry
Optimal working dilutions must be determined by the end user.
1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000-1:100,000 for western blotting with chemiluminescent substrates.
1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.
1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry
Optimal working dilutions must be determined by the end user.
This Goat anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Rabbit IgG.
外形
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide
RECONSTITUTION:
Reconstitute with 1.5 mL sterile distilled water.
RECONSTITUTION:
Reconstitute with 1.5 mL sterile distilled water.
储存及稳定性
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C with or without aliquotting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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