推荐产品
生物来源
goat
质量水平
偶联物
peroxidase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
种属反应性
rat
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
山羊抗大鼠IgG亲和纯化抗体,HRP偶联。
特异性
对大鼠IgG重链和轻链具有特异性。
应用
使用经验证可用于ELISA、IH&WB的山羊抗大鼠IgG抗体,HRP偶联物检测大鼠IgG。
免疫组化:
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
质量
酶联免疫吸附测定常规评估。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
外形
冻干。缓冲液=0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/mL BSA。
用2 mL无菌蒸馏水复溶。
用2 mL无菌蒸馏水复溶。
储存及稳定性
将冻干产品在2-8°C下保存长达12个月。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。 要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到50%,然后以未稀释的等分试样置于-20°C下可保存长达12个月。 请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告: 使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。 要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到50%,然后以未稀释的等分试样置于-20°C下可保存长达12个月。 请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告: 使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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