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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
rat
Citations:
75
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
产品名称
山羊抗大鼠IgG抗体,HRP偶联, Chemicon®, from goat
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Analysis Note
酶联免疫吸附测定常规评估。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
Application
使用经验证可用于ELISA、IH&WB的山羊抗大鼠IgG抗体,HRP偶联物检测大鼠IgG。
免疫组化:
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
对大鼠IgG重链和轻链具有特异性。
General description
山羊抗大鼠IgG亲和纯化抗体,HRP偶联。
Physical form
冻干。缓冲液=0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/mL BSA。
用2 mL无菌蒸馏水复溶。
用2 mL无菌蒸馏水复溶。
Preparation Note
将冻干产品在2-8°C下保存长达12个月。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。 要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到50%,然后以未稀释的等分试样置于-20°C下可保存长达12个月。 请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告: 使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。 要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到50%,然后以未稀释的等分试样置于-20°C下可保存长达12个月。 请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告: 使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Mark A Csandl et al.
Drug metabolism and disposition: the biological fate of chemicals, 44(6), 857-866 (2016-04-14)
Active efflux of both drugs and organic anion metabolites is mediated by the multidrug resistance proteins (MRPs). MRP1 (ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and MRP4 (ABCC4) have partially overlapping substrate specificities and all transport 17β-estradiol 17-(β-d-glucuronide) (E217βG). The cysteinyl leukotriene
Felix Wiedmann et al.
Molecular biology of the cell, 30(12), 1425-1436 (2019-04-11)
Two pore-domain potassium (K2P) channels mediate potassium background currents that stabilize the resting membrane potential and facilitate action potential repolarization. In the human heart, hK2P17.1 channels are predominantly expressed in the atria and Purkinje cells. Reduced atrial hK2P17.1 protein levels
Counteraction of tetherin antiviral activity by two closely related SIVs differing by the presence of a Vpu gene.
Nikovics, K; Dazza, MC; Ekwalanga, M; Mammano, F; Clavel, F; Saragosti, S
Testing null
Jing Zhu et al.
Scientific reports, 6, 28861-28861 (2016-06-29)
Ethoxyquin was recently identified as a neuroprotective compound against toxic neuropathies and efficacy was demonstrated against paclitaxel-induced neurotoxicity in vivo. In this study we examined the efficacy of ethoxyquin in preventing neurotoxicity of cisplatin in rodent models of chemotherapy-induced peripheral
N V Andreeva et al.
Cell stress & chaperones, 21(4), 727-733 (2016-04-20)
Heat shock proteins including the major stress protein HSP70 support intracellular homeostasis and prevent protein damage after a temperature increase and other stressful environmental stimuli, as well as during aging. We have shown earlier that prolonged administration of recombinant human
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