推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性（根据同源性预测）
primate (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pSer293)
基因信息
human ... PDHA1(5160)
一般描述
在许多生物体内，丙酮酸脱氢酶（PDH）复合物在有氧途径中催化丙酮酸整体不可逆地转化为乙酰辅酶A和CO2。这种复合物也是心脏底物选择的关键调节因子。它包含丙酮酸脱氢酶（E1或I型PDHE1-A）、二氢硫辛酰胺乙酰转移酶（E2）和硫辛酰胺脱氢酶（E3）三种酶组分的多个拷贝。PDH通过丙酮酸脱氢酶激酶（PDK）介导的磷酸化和反馈抑制调节。
特异性
该抗体可识别在Ser293处磷酸化的I型PDHE1-A。
免疫原
KLH偶联线性肽，对应于在Ser293处磷酸化的人I型PDHE1-A。
表位：磷酸化Ser293
应用
免疫细胞化学分析：代表性批次抗体的1:500稀释液在未经处理和经DCA处理的HEK293细胞中检测到磷酸化PDHE1-A I型（Ser293）。
可使用这种磷酸化I型PDHE1-A（Ser293）抗体检测磷酸化I型PDHE1-A（Ser293），该抗体经验证可用于WB、IC & IP。
研究子类别
葡萄糖/糖原代谢
葡萄糖/糖原代谢
研究类别
代谢
代谢
质量
通过蛋白质印迹法对未经处理和经DCA处理的HEK293细胞裂解液进行了评估。
蛋白质印迹分析：该抗体的1:10,000稀释液在未经处理和经DCA处理的HEK293细胞裂解液中检测到磷酸化I型PDHE1-A（Ser293）。
蛋白质印迹分析：该抗体的1:10,000稀释液在未经处理和经DCA处理的HEK293细胞裂解液中检测到磷酸化I型PDHE1-A（Ser293）。
目标描述
观测值〜43 kDa
外形
亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
储存及稳定性
自收到之日起，在2-8°C条件下可稳定保存1年。
分析说明
对照
未经处理和经DCA处理的HEK293细胞裂解液
未经处理和经DCA处理的HEK293细胞裂解液
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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