推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性（根据同源性预测）
mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
技术
immunocytochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pSer300)
基因信息
human ... PDHA1(5160)
一般描述
在许多生物体中，丙酮酸脱氢酶复合体除了作为心脏底物选择的关键调节因子外，还在有氧能量生成途径中催化丙酮酸到乙酰辅酶a和二氧化碳的总体不可逆转化。它含有三种酶成分的多个副本：丙酮酸脱氢酶（E1）、二氢脂酰胺乙酰转移酶（E2）和脂酰胺脱氢酶（E3）。PDH通过丙酮酸脱氢酶激酶（PDK）介导的磷酸化和反馈抑制调节。
特异性
该抗体可识别在Ser300磷酸化的PDHE1-A 1型。
免疫原
对应于在Ser300磷酸化的1型人PDHE1-A的KLH偶联线性肽。
表位：磷酸化Ser300
应用
免疫细胞化学分析： 代表性批次的1:500稀释液在二氯乙酸未处理和已处理的HEK 293细胞中检测到PDHE1-A 1型。
该抗磷酸化PDHE1-A 1型（Ser300）抗体经验证可用于在WB、IC中检测磷酸化PDHE1-A 1型（Ser300）。
质量
已通过蛋白质印迹在二氯乙酸未处理和已处理的NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析：0.5 µg/mL该抗体在10 µg二氯乙酸未处理和已处理的HEK 293细胞裂解液中检测到PDHE1-A 1型。
蛋白质印迹分析：0.5 µg/mL该抗体在10 µg二氯乙酸未处理和已处理的HEK 293细胞裂解液中检测到PDHE1-A 1型。
目标描述
观测值〜43 kDa
分析说明
对照
二氯乙酸未处理和已处理的HEK 293细胞裂解液
二氯乙酸未处理和已处理的HEK 293细胞裂解液
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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