Anti-AIP1/Alix Antibody

Programmed cell death 6-interacting protein (UniProt: Q9WU78; also known as ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2) is encoded by the Pdcd6ip (also known as Aip1, Alix) gene (Gene ID: 18571) in murine species. AIP1 is a class E VPS cytoplasmic, ubiquitously expressed protein that is involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intra-lumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. AIP1 interacts with ALG-2 (apoptosis-linked gene-2) to regulate cell death. This interaction is strictly dependent on calcium. AIP1 is considered to be essential for the maintenance of fibroblast morphology and has been shown to play a role in the regulation of both apoptosis and cell proliferation. AIP1 is also known to regulate exosome biogenesis in concert with SDC1/4 and SDCBP. AIP1 can undergo phosphorylated on tyrosine residues by activated platelet-derived growth factor receptor-beta (PDGFRB).

This polyclonal antibody specifically detects AIP1 in Jurkat cells. It is likely to react with isoforms 1, 2, and 3.

GST-tagged recombinant fragment corresponding to 479 amino acids from the C-terminal half of mouse AIP1/Alix.

Detect Programmed cell death 6-interacting protein using this rabbit polyclonal Anti-AIP1/Alix, Cat. No. ABC40 that has been tested in Immunocytochemistry and Western blotting.

Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected AIP1/Alix in HeLa and NIH3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.

~95 kDa observed; 96.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 20 mM Tris-HCl, pH 7.2, 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

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