AB10423
Anti-Phospho- Bad (Ser75) Antibody
from rabbit
别名:
BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-associated agonist of cell death, BCL2-binding component 6, BCL2-binding protein, Bcl-2-binding component 6, Bcl-2-like 8 protein, Bcl-XL/Bcl-2-associated death promoter
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
mouse, rat
Application:
western blot
Technique(s):
western blot: suitable
Citations:
1
Uniprot accession no.:
产品名称
Anti-Phospho- Bad (Ser75) Antibody, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer75)
Quality Level
Gene Information
human ... BAD(572)
Physical form
Purified immunoglobulins in PBS with 0.09% NaN3.
Format: Purified
Protein G followed by IAP
Analysis Note
Control
Western Blot : RAW264.7/LPS lysate.
Western Blot : RAW264.7/LPS lysate.
Western Blot Analysis: 1:250 dilution of this antibody detected phospho-Bad (Ser75) in lysate from LPS-stimulated RAW264.7 cells.
Application
Anti-Phospho-Bad (Ser75) Antibody detects level of Phospho-Bad (Ser75) & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Biochem/physiol Actions
Other species have not been tested.
This antibody reacts with the 18 kDa phospho-Bad (Ser75) protein.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
18 kDa
Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
Immunogen
This antibody is generated in rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS75 of human Bad.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Preparation Note
Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Xiaolong Wang et al.
Cell and tissue research, 362(2), 317-330 (2015-06-17)
Growth/differentiation factor-15 (GDF-15) is a distant member of the transforming growth factor beta (TGF-β) superfamily. It is widely distributed in the nervous system, where it has been shown to play an important role in neuronal maintenance. The present study investigates
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