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70622-M

Sigma-Aldrich

Tuner Competent Cells - Novagen

Escherichia coli, rod shaped

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About This Item

UNSPSC代码:
41106202
价格与库存信息目前不能提供

产品名称

Tuner Competent Cells - Novagen, Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

生物来源

Escherichia coli

质量水平

100

制造商/商品名称

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

transformation efficiency: >2×106 cfu/μg

运输

dry ice

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Tuner Competent Cells - Novagen Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

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Tuner(DE3)pLysS Competent Cells - Novagen Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.

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Tuner(DE3)pLysS Competent Cells - Novagen

Tuner (DE3) Competent Cells - Novagen® Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

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Tuner (DE3) Competent Cells - Novagen®

Rosetta 2(DE3)pLacI Competent Cells - Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

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Rosetta 2(DE3)pLacI Competent Cells - Novagen

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice, wet ice

shipped in

dry ice

一般描述

Genotype: F–ompT hsdSB (rB– mB–) gal dcm lacY1





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

组分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

法规信息

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