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生物来源
human plasma
质量水平
表单
liquid
制造商/商品名称
Novagen®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
技术
protein extraction: suitable
适用性
suitable for additive or modifier in the separation of proteins or peptides
应用
life science and biopharma
运输
wet ice
储存温度
−20°C
一般描述
限制级凝血酶是一种高度纯化的制剂,能够特异性切割用适当载体产生的靶蛋白。该制剂通过融合蛋白活性功能测试,不含可检测的污染蛋白酶。由经认证测试对HBsAg以及HIV和HCV抗体阴性的人血浆制制备而成。凝血酶以50%甘油溶液的形式提供,包括10倍凝血酶裂解缓冲液和裂解控制蛋白。
组分
•50 U凝血酶
•1 ml10X 凝血酶裂解缓冲液
•2 ml1X 凝血酶稀释/储存缓冲液
•10 µg裂解对照蛋白
•1 ml10X 凝血酶裂解缓冲液
•2 ml1X 凝血酶稀释/储存缓冲液
•10 µg裂解对照蛋白
警告
毒性:多个毒性值,请参阅MSDS(O)
单位定义
一个单位定义为:在200 µl反应中,在20°C下,在16小时内裂解1 mg融合蛋白所需的酶量,该反应含有20 mM Tris-HCl pH 8.4、150 mM NaCl、2.5 mM CaCl₂、50 µg融合蛋白。
制备说明
由经认证测试对HBsAg以及HIV和HCV抗体阴性的人血浆制制备而成。
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one
商品
Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.
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