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69284

Sigma-Aldrich

NovaBlue(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

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选择尺寸

100 G
¥770.90

¥770.90


国内现货,预计发货时间2025年4月25日详情



选择尺寸

变更视图
100 G
¥770.90

About This Item

UNSPSC代码:
41106202
NACRES:
NA.81

¥770.90


国内现货,预计发货时间2025年4月25日详情


产品名称

NovaBlue(DE3) Competent Cells - Novagen, NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, DE3 lysogen cannot be used for the blue/white screening of recombinant plasmids

生物来源

Escherichia coli

质量水平

制造商/商品名称

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

transformation efficiency: >1×108 cfu/μg

运输

dry ice

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此商品
31277202185V800125
form

solid

form

solid

form

crystals or chunks

form

solid

assay

≥97%, 97.0-102.0% (KT)

assay

98-102%

assay

-

assay

≥98%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-

vapor pressure

40 mmHg ( 0 °C)

vapor pressure

40 mmHg ( 0 °C)

vapor pressure

40 mmHg ( 0 °C)

vapor pressure

40 mmHg ( 0 °C)

anion traces

nitrate (NO3-): ≤0.01%, sulfate (SO42-): ≤0.007%

anion traces

nitrate (NO3-): ≤100 mg/kg, sulfate (SO42-): ≤50 mg/kg

anion traces

-

anion traces

sulfate (SO42-): ≤0.03%

cation traces

Ni: ≤0.15%, Zn: ≤0.05%, Fe: ≤0.005%

cation traces

Ca: ≤50 mg/kg, Cu: ≤20 mg/kg, Fe: ≤50 mg/kg, K: ≤50 mg/kg, Mg: ≤10 mg/kg, Mn: ≤50 mg/kg, Na: ≤100 mg/kg, Ni: ≤500 mg/kg, Pb: ≤5 mg/kg, Zn: ≤25 mg/kg

cation traces

-

cation traces

Fe: ≤0.01%

一般描述

NovaBlue(DE3) Competent Cells is a K-12 strain with high transformation efficiency and recA endA mutations. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor encoded by the F episome.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

组分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlNovaBlue(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

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    Gillian Dunphy et al.
    Molecular cell, 71(5), 745-760 (2018-09-08)
    DNA damage can be sensed as a danger-associated molecular pattern by the innate immune system. Here we find that keratinocytes and other human cells mount an innate immune response within hours of etoposide-induced DNA damage, which involves the DNA sensing

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