product name
NovaBlue(DE3) Competent Cells - Novagen, NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, DE3 lysogen cannot be used for the blue/white screening of recombinant plasmids
生物来源
Escherichia coli
质量水平
制造商/商品名称
Novagen®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
transformation efficiency: >1×108 cfu/μg
运输
dry ice
储存温度
−70°C
一般描述
NovaBlue(DE3) Competent Cells is a K-12 strain with high transformation efficiency and recA endA mutations. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor encoded by the F episome.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
组分
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlNovaBlue(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlNovaBlue(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
Gillian Dunphy et al.
Molecular cell, 71(5), 745-760 (2018-09-08)
DNA damage can be sensed as a danger-associated molecular pattern by the innate immune system. Here we find that keratinocytes and other human cells mount an innate immune response within hours of etoposide-induced DNA damage, which involves the DNA sensing
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