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Merck
CN
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文件

69053

Sigma-Aldrich

BLR(DE3)感受态细胞- Novagen

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

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UNSPSC代码:
41106202
NACRES:
NA.81

生物来源

Escherichia coli

质量水平

100

制造商/商品名称

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

transformation efficiency: >2×106 cfu/μg

运输

dry ice

储存温度

−70°C

相关类别

一般描述

基因型：F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)





本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
BLR是BL21的recA-衍生物，可提高质粒单体产量，并有助于稳定含有重复序列，或其产物可能导致DE3原噬菌体丢失的靶质粒（1,2）。

DE3表明宿主是λDE3的溶酶原，因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
BLR是BL21的recA衍生物，可提高质粒单体产量，并有助于稳定含有重复序列的靶质粒。

组分

0.4 ml1 ml成分

•2 × 0.2 ml5 × 0.2 mlBLR(DE3)感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng测试质粒

警告

毒性：多个毒性值，请参阅MSDS（O）

其他说明

1.A. Roca (U. of Wisconsin), personal
communication.

2.Studier, F. W. (1991) J. Mol.Biol. 219,
37-44.

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

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