product name
B834(DE3)感受态细胞 - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
生物来源
Escherichia coli
制造商/商品名称
Novagen®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
transformation efficiency: >2×106 cfu/μg
运输
dry ice
储存温度
−70°C
一般描述
B834是BL21（1）的亲本菌株。这些缺乏蛋白酶的宿主是蛋氨酸营养缺陷型，允许用35S蛋氨酸和硒代蛋氨酸对目标蛋白质进行高比活性标记，用于结晶学（2）。
DE3表明宿主是λDE3的溶酶原，因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
DE3表明宿主是λDE3的溶酶原，因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
基因型：F-ompT hsdSB(rB- mB-) gal dcm met (DE3)
本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
本产品含有转基因生物（GMO）。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
B834是BL21的亲本菌株。这些宿主是蛋氨酸营养缺陷型，允许用35S蛋氨酸和硒代蛋氨酸对目标蛋白质进行高比活性标记，以用于结晶学。
组分
0.4 ml1 ml成分
•2 × 0.2 ml5 × 0.2 mlB834（DE3）感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng测试质粒
•2 × 0.2 ml5 × 0.2 mlB834（DE3）感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng测试质粒
警告
毒性：多个毒性值，请参阅MSDS（O）
其他说明
1. Wood, W. B. (1966) J. Mol.Biol. 16, 118–133.
2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
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