description
RTECS - MD0905000
Quality Level
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
color
clear colorless
CMC
50 - 300 μM
solubility
water: 25 mg/mL, aqueous buffer: soluble
density
1.06 g/mL
application(s)
(Isolating membrane proteins)
shipped in
ambient
storage temp.
15-25°C
SMILES string
O(CCOc1ccc(cc1)CCCCCCCCC)CCO
InChI
1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3
InChI key
BLXVTZPGEOGTGG-UHFFFAOYSA-N
General description
一种用于分离和纯化功能性膜蛋白复合物和双向（2D）凝胶电泳的非离子表面活性剂。与NP-40和TRITON ™X-100洗涤剂（目录号648462）的用法相似。
Application
NP-40 Alternative has been used:
- as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
- as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
- for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).
Other Notes
Ripoche, P., et al. 1982.Biochim.Biophys.Acta693, 497.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
毒性：刺激性（B）
存储类别
11 - Combustible Solids
flash_point_f
503.6 °F - Pensky-Martens closed cup - (own results)
flash_point_c
262 °C - Pensky-Martens closed cup - (own results)
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
法规信息
监管及禁止进口产品
此项目有
Gustav Roder et al.
European journal of immunology, 39(10), 2682-2694 (2009-09-04)
Tapasin (Tpn) is an ER chaperone that is uniquely dedicated to MHC-I biosynthesis. It binds MHC-I molecules, integrates them into peptide-loading complexes, and exerts quality control of the bound peptides; only when an "optimal peptide" is bound will the MHC-I
Matthew R Miller et al.
Journal of biological methods, 2(3), e23-e23 (2015-10-13)
Over the past two decades, researchers have struggled to efficiently express foreign DNA in primary macrophages, impeding research progress. The applications of lipofection, electroporation, microinjection, and viral-mediated transfer typically result in disruptions in macrophage differentiation and function, low expression levels
Ying Zhang et al.
Cancer research, 79(19), 4994-5007 (2019-08-09)
Human astrocytomas and oligodendrogliomas are defined by mutations of the metabolic enzymes isocitrate dehydrogenase (IDH) 1 or 2, resulting in the production of the abnormal metabolite D-2 hydroxyglutarate. Here, we studied the effect of mutant IDH on cell proliferation and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 492016-100ML | 07790788050832 |
| 492016-1000ML | 04055977273489 |
| 492016-500ML | 04055977273496 |