产品名称
Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate, liquid, clone HP6043, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6043, monoclonal
form
liquid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.
Application
Enzyme Immunoassay (1:1000, see application references)
Disclaimer
Toxicity: Standard Handling (A)
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 10 mg/ml BSA.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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