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Merck
CN

16-232

抗-α-微管蛋白抗体，克隆DM1A，Alexa Fluor™ 488结合物

clone DM1A, Upstate®, from mouse

别名:

Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗-α-微管蛋白抗体，克隆DM1A，Alexa Fluor™ 488结合物, clone DM1A, Upstate®, from mouse

biological source

mouse

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

rat, mouse, guinea pig, human, pig, bovine, avian

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_006000

UniProt accession no.

Q71U36

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TUBA1A(7846)

相关类别

Analysis Note

对照
阴性对照：目录号16-240，正常小鼠IgG，Alexa Fluor™ 488-偶联物。
通过流式细胞术进行常规评估。

流式细胞术：
0.2 μg该批次在固定和透化的大鼠L6，Jurkat和A431细胞中检测到α-微管蛋白。

Application

抗α微管蛋白抗体，克隆DM1A，Alexa Fluor 488偶联物是一种抗α微管蛋白的抗体，用于FC、IC和IF & WB。
蛋白质印迹分析：
2 µg/mL的先前批次在A431细胞的RIPA裂解物中检测到α-微管蛋白。

免疫细胞化学：
2 µg/mL先前批次显示了HeLa细胞中α-微管蛋白的阳性免疫染色。

Biochem/physiol Actions

识别α-微管蛋白，克隆DM1A，Mr 50-60 kDa。

General description

50-60 kDa
微管蛋白是细胞骨架的主要成分，形成对细胞结构、迁移、细胞分裂和细胞内运输至关重要的微管。微管蛋白至少有α、β和γ三种。每种分子量都约为55 kDa。微管是由交替的α和β亚基组成的大型结构（直径300埃）。阻止微管形成的化合物，例如紫杉醇和长春花生物碱，可抑制细胞分裂，并且是有效的抗癌剂。

Immunogen

纯化人脑α-微管蛋白。 该抗体识别的表位已定位到氨基酸426-450（AALEKDYEEVGVDSV-EGEGEEEGEE）。
表位：该表位已定位到氨基酸426-450（AALEKDYEEVGVDSV-EGEGEEEGEE）。

Other Notes

浓度：请参考批次特异性浓缩物的检验报告。

Physical form

Alexa Fluor™ 488偶联，纯化的小鼠单克隆IgG1溶于含有1%BSA，0.05%吐温-20、0.05%叠氮化钠的PBS的缓冲液中。
在2-8°C为液体形式。

Preparation Note

自收到之日起，在2-8°C条件下可稳定保存1年。
不得冷冻。 不要储存稀释的材料。
为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Simon Schneider et al.
eLife, 12 (2023-11-28)
Cylicins are testis-specific proteins, which are exclusively expressed during spermiogenesis. In mice and humans, two Cylicins, the gonosomal X-linked Cylicin 1 (Cylc1/CYLC1) and the autosomal Cylicin 2 (Cylc2/CYLC2) genes, have been identified. Cylicins are cytoskeletal proteins with an overall positive
Dhanusha A Nalawansha et al.
Journal of the American Chemical Society, 144(12), 5594-5605 (2022-03-22)
Targeted protein degradation (TPD) by PROTACs is a promising strategy to control disease-causing protein levels within the cell. While TPD is emerging as an innovative drug discovery paradigm, there are currently only a limited number of E3 ligase:ligand pairs that
Naila Umer et al.
Frontiers in cell and developmental biology, 9, 749559-749559 (2021-12-07)
Profilins (PFNs) are key regulatory proteins for the actin polymerization in cells and are encoded in mouse and humans by four Pfn genes. PFNs are involved in cell mobility, cell growth, neurogenesis, and metastasis of tumor cells. The testes-specific PFN3
Wei-Ting Yueh et al.
Development (Cambridge, England) (2021-12-15)
Aneuploidy is frequently observed in oocytes and early embryos, begging the question of how genome integrity is monitored and preserved during this critical period. SMC3 is a subunit of the cohesin complex that supports genome integrity, but its role in
Yin-Wei Kuo et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5533-5541 (2019-03-07)
The remodeling of the microtubule cytoskeleton underlies dynamic cellular processes, such as mitosis, ciliogenesis, and neuronal morphogenesis. An important class of microtubule remodelers comprises the severases-spastin, katanin, and fidgetin-which cut microtubules into shorter fragments. While severing activity might be expected
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