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16-232

抗-α-微管蛋白抗体，克隆DM1A，Alexa Fluor^™ 488结合物

Name: 抗-α-微管蛋白抗体，克隆DM1A，Alexa Fluor<SUP>™</SUP> 488结合物
Brand: MM

clone DM1A, Upstate^®, from mouse

别名:

Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific

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About This Item

UNSPSC代码:

12352203

eCl@ss:

32160702

NACRES:

NA.41

生物来源

mouse

质量水平

100

偶联物

ALEXA FLUOR^™ 488

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

DM1A, monoclonal

种属反应性

rat, mouse, guinea pig, human, pig, bovine, avian

制造商/商品名称

Upstate^®

技术

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

NP_006000

UniProt登记号

Q71U36

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... TUBA1A(7846)

一般描述

微管蛋白是细胞骨架的主要成分，形成对细胞结构、迁移、细胞分裂和细胞内运输至关重要的微管。微管蛋白至少有α、β和γ三种。每种分子量都约为55 kDa。微管是由交替的α和β亚基组成的大型结构（直径300埃）。阻止微管形成的化合物，例如紫杉醇和长春花生物碱，可抑制细胞分裂，并且是有效的抗癌剂。

特异性

识别α-微管蛋白，克隆DM1A，Mr 50-60 kDa。

免疫原

纯化人脑α-微管蛋白。该抗体识别的表位已定位到氨基酸426-450（AALEKDYEEVGVDSV-EGEGEEEGEE）。

表位：该表位已定位到氨基酸426-450（AALEKDYEEVGVDSV-EGEGEEEGEE）。

应用

抗α微管蛋白抗体，克隆DM1A，Alexa Fluor 488偶联物是一种抗α微管蛋白的抗体，用于FC、IC和IF & WB。

蛋白质印迹分析：
2 µg/mL的先前批次在A431细胞的RIPA裂解物中检测到α-微管蛋白。

免疫细胞化学：
2 µg/mL先前批次显示了HeLa细胞中α-微管蛋白的阳性免疫染色。

质量

通过流式细胞术进行常规评估。

流式细胞术：
0.2 μg该批次在固定和透化的大鼠L6，Jurkat和A431细胞中检测到α-微管蛋白。

目标描述

50-60 kDa

外形

Alexa Fluor^™ 488偶联，纯化的小鼠单克隆IgG1溶于含有1%BSA，0.05%吐温-20、0.05%叠氮化钠的PBS的缓冲液中。
在2-8°C为液体形式。

储存及稳定性

自收到之日起，在2-8°C条件下可稳定保存1年。
不得冷冻。不要储存稀释的材料。
为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。

分析说明

对照
阴性对照：目录号16-240，正常小鼠IgG，Alexa Fluor^™ 488-偶联物。

其他说明

浓度：请参考批次特异性浓缩物的检验报告。

法律信息

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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抗-α-微管蛋白抗体，克隆DM1A，Alexa Fluor™ 488结合物

clone DM1A, Upstate®, from mouse