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1.52258

Chromolith^® WP 300 Protein A (monolithic) HPLC Columns

Name: Chromolith<SUP>&#174;</SUP> WP 300 Protein A (monolithic) HPLC Columns
Brand: MM

L × I.D. 25 mm x 4.6 mm HPLC Column

别名:

Chromolith^® WP 300 Protein A 25-4.6 HPLC column

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About This Item

UNSPSC代码:

41115700

NACRES:

SB.52

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Sigma-Aldrich

AP502P

山羊抗人Igκ链抗体，HRP偶联，吸附物种

产品名称

Chromolith^® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

物料

PEEK column

产品线

Chromolith^®

特点

endcapped: no
endcapped: no

包装

1 ea of

参数

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

技术

HPLC: suitable
HPLC: suitable
LC/MS: suitable

色谱柱长度 × 内径

2.5 cm × 4.6 mm

表面积

120 m²/g

基质

monolithic silica
monolithic silica

基质活性基团

Protein A bonding phase
Protein A bonding phase

孔径

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

工作pH范围

1.5-7.5

工作pH值

1.5-7.5

分离技术

affinity

储存温度

2-8°C
2-8°C

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一般描述

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith^® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith^® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

Chromolith^® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith^® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

法律信息

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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商品

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

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Chromolith^® WP 300 Protein A (monolithic) HPLC Columns