推荐产品
product name
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
物料
PEEK column
质量水平
Agency
suitable for USP L8
产品线
Chromolith®
特点
endcapped: no
包装
1 ea of
标记范围
5% Carbon loading
参数
200 bar pressure
45 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
色谱柱长度 × 内径
10 cm × 4.6 mm
基质
monolithic silica
基质活性基团
NH2 bonding phase
孔径
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
分离技术
hydrophilic interaction (HILIC)
normal phase
reversed phase
储存温度
no temp limit
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相关类别
一般描述
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
分析说明
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
法律信息
商品
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
实验方案
Separation of Xylose; Fructose; Glucose; Saccharose
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