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产品名称
Chromolith® HighResolution RP-18 封端, L × I.D. 25 mm x 4.6 mm HPLC column
物料
PEEK column
质量水平
Agency
suitable for ISO/CEN 6304.09
suitable for USP L1
产品线
Chromolith®
特点
endcapped
endcapped
包装
1 ea of
标记范围
15% Carbon loading
18% Carbon loading
参数
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
技术
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
色谱柱长度 × 内径
2.5 cm × 4.6 mm
表面积
250 m2/g × 250 m2/g
基质
highly porous
monolithic silica
monolithic silica
基质活性基团
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
孔径
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
工作pH范围
2-7.5
2-7.5
分离技术
reversed phase
储存温度
no temp limit
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一般描述
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.
Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparableto a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for very rapid separations on any HPLC System and rapid method development.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
分析说明
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 110000
Capacity factor (Anthracene): 3.3 - 4.6
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (anthracene/progesterone): 1.85 - 2.35
法律信息
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商品
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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