纯化方式
immobilized metal affinity chromatography (IMAC)
质量水平
技术
activity assay: suitable
UniProt登记号
基因信息
human ... EIF2AK3(9451)
一般描述
N-terminal, 6His-tagged, recombinant human PEK, amino acids 536-end.
包装
Also available in 250 µg size - call for pricing and availability and reference catalog number 14-916M when ordering the 250 µg size.
质量
Routinely evaluated by phosphorylation of RSRSRSRSRSRSRSR. For Specific Activity Data, refer to the Certificate of Analysis for individual lots of this enzyme.
物理属性
71 kDa
外形
50 mM Tris-HCl, pH 7.5, 0.3 M NaCl, 0.1 mM EGTA, 0.03 % Brij-35, 270 mM sucrose, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200 μM PMSF
储存及稳定性
6 months at -70°C
其他说明
FUNCTION: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1 (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Ubiquitous. A high level expression is seen in secretory tissues.
PTM: Autophosphorylated. N-glycosylated (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 1 protein kinase domain.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Ubiquitous. A high level expression is seen in secretory tissues.
PTM: Autophosphorylated. N-glycosylated (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 1 protein kinase domain.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语:
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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