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Merck
CN

1.11350

Bactident™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

别名:

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

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NACRES:
NA.84
UNSPSC Code:
41106200

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产品名称

Bactident™ Kovac′s Indole reagent, Available in a practical dropper bottle, for Escherichia coli

description

Bactident™ E. coli 1.13303

form

liquid (ready-to-use)

shelf life

210 days

feature

ready-to-use

composition

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

packaging

pkg of (1 x 30 ml, Plastic bottle)

technique(s)

microbe id | specific enzyme detection: suitable

pH

<1 (20 °C in H2O)

transition temp

flash point 36 °C

density

0.92 g/cm3 at 20 °C

application(s)

environmental
food and beverages

storage temp.

2-8°C

suitability

Enterobacter spp.
Escherichia coli

Quality Level

100

相关类别

Analysis Note

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Application

Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

General description

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Other Notes

Replaces: 1.11350.0007

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

96.8 °F

flash_point_c

36 °C

法规信息

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Cultivation of Shigella

Shigella spp. are predominantly connected to humans and are rarely isolated from other animals, with the exception of primates.

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

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