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Merck
CN
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安全信息

1.11350

Millipore

Bactident Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

别名:

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

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About This Item

UNSPSC代码:
41106200
NACRES:
NA.84

质量水平

描述

Bactident™ E. coli 1.13303

形式

liquid (ready-to-use)

保质期

210 days

特点

ready-to-use

组成

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

包装

pkg of (1 x 30 ml, Plastic bottle)

技术

microbe id | specific enzyme detection: suitable

pH值(酸碱度)

<1 (20 °C in H2O)

转变温度

flash point 36 °C

密度

0.92 g/cm3 at 20 °C

应用

environmental
food and beverages

储存温度

2-8°C

适用性

Enterobacter spp.
Escherichia coli

一般描述

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

应用

Bactident Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

联系

Replaces: 1.11350.0007

分析说明

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

法律信息

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

96.8 °F

闪点(°C)

36 °C

法规信息

监管及禁止进口产品

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