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Merck
CN
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文件

1.10765

Millipore

EC (Escherichia coli) broth

GranuCult® prime, suitable for microbiology, for Escherichia coli

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别名:
E. coli selective medium, Escherichia coli broth, EC broth
UNSPSC代码:
41106213
NACRES:
NA.74

Agency

FDA-BAM
ISO 7251

质量水平

200

形式

medium granules (dehydrated (DCM))

包装

pkg of 500 g

制造商/商品名称

GranuCult® prime

储存条件

dry media (Tightly closed.)

技术

microbiological culture: suitable

颜色

beige

pH值（酸碱度）

6.7-7.1 (25 °C, 37 g/L in H2O, after autoclaving)

溶解性

74 g/L

应用

food and beverages
microbiology

储存温度

15-25°C

适用性

Escherichia coli
Escherichia spp.

相关类别

一般描述

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria. The lactose content of this medium favors the growth of lactose-positive bacteria, especially coliform bacteria, and E. coli. The bile salts inhibit the growth of Gram-positive bacteria or microorganisms that are not adapted to the intestinal environment. Lactose-positive bacteria metabolize lactose with gas formation.

应用

Escherichia coli broth is suitable for the selective enrichment, enumeration, and confirmation of coliform bacteria from food and animal feed, and other materials.

特点和优势

  • GranuCult® prime offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

分析说明

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1E+04
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1E+04
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Escherichia coli: 24 ± 2 hours at 44 ± 1 °C
other teststrains: 48 ± 2 hours at 44 ± 1 °C

附注

We offer two media types: the superior granulated GranuCult® prime and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 2

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