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1.10690

Millipore

GranuCult® plus DEV Lactose peptone broth

for coliforms, suitable for microbiology

别名:

Coliform enrichment broth, Lactose Peptone Broth DEV

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About This Item

UNSPSC代码:
41106213
NACRES:
NA.74

表单

medium granules (dehydrated (DCM))

质量水平

200

包装

pkg of 500 g

制造商/商品名称

GranuCult® plus

储存条件

protect from light (Keep dry and tightly closed)

技术

microbiological culture: suitable

颜色

violet

pH值（酸碱度）

7.0 (37 °C, 25 g/L in H2O, after autoclaving)

溶解性

25 g/L at 100 °C, clear

堆积密度

700 kg/m3

应用

food and beverages
water monitoring
microbiology

储存温度

15-25°C

适用性

coliforms

相关类别

一般描述

GranuCult® plus DEV Lactose peptone broth is suited for the enrichment and determination of the titer of coliform bacteria in the bacteriological analysis of water according to the German standard methods for the examination of water and the previous German regulations from 1990.Casein peptone and soya peptone provide nitrogen, vitamins, minerals, and amino acids for growth, while sodium chloride ensures osmotic balance. Lactose is a carbohydrate source. The pH indicator bromocresol purple is yellow below pH 5.3 (acidic) and purple above pH 6.7 (alkaline). The fermentation of lactose and the consequent acid production changes the color of the medium to yellow.

应用

GranuCult® plus DEV Lactose peptone broth is suitable for the enrichment of coliforms in the bacteriological analysis of water.

特点和优势

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

分析说明

Appearance (clearness): clear
Appearance (colour): violet
pH-value (25 °C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): medium to very good
Growth (Enterococcus faecalis ATCC 11700): medium to very good
Colour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013)): +
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Colour change to yellow (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Colour change to yellow (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Colour change to yellow (Enterococcus faecalis ATCC 11700): +/-
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Gas (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Gas (Enterococcus faecalis ATCC 11700): -
Incubation: 24 hrs.; 35 °C; aerobic.

其他说明

  • Do not use clumped or discolored medium.
  • The prepared broth is clear and purple.
  • The pH for double-concentrated can differ from single-concentrated DEV Lactose Peptone broth.

附注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 2

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