1.10316
Fractogel EMD TMAE Hicap (M)
strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
别名:
Fractogel® EMD TMAE Hicap (M)
产品名称
Fractogel EMD TMAE Hicap (M), strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
ligand
(Trimethylammoniumethyl)
Quality Level
form
resin
specific analyte(s)
MAbs
parameter
180 cm/hr flow rate
8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
180 mg binding capacity (BSA/mg of resin)
transition temp
flash point 35 °C (calculated)
density
1.430 g/cm3 at 20 °C
bulk density
1000 kg/m3
application(s)
recombinant protein
separation technique
anion exchange
strong anion exchange
storage temp.
2-30°C
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Analysis Note
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 160 - 200 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin
Features and Benefits
- Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
- Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
General description
Packaging
- 1.10316.0100: Fractogel® EMD TMAE Hicap (M) Resin 100ml
- 1.10316.0010: Fractogel® EMD TMAE Hicap (M) Resin 10ml
- 1.10316.0500: Fractogel® EMD TMAE Hicap (M) Resin 500ml
- 1.10316.5000: Fractogel® EMD TMAE Hicap (M) Resin 5L
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
95.0 °F
flash_point_c
35 °C
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