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Merck
CN
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1.10266

Millipore

GranuCult® prime Lauryl Sulfate broth

according to ISO 4831, ISO 7251, FDA-BAM, suitable for microbiology, for Escherichia coli

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别名:
Coliform selective medium, Lauryl Sulfate broth (Granulated)
UNSPSC代码:
41106213
NACRES:
NA.74

Agency

SMWWPart 9221
FDA-BAM
GB 4789.38-2012
ISO 7251
according to ISO 4831

质量水平

200

形式

medium granules (dehydrated (DCM))

包装

pkg of 5 kg
pkg of 500 g

制造商/商品名称

GranuCult® prime

储存条件

dry media (Keep tightly closed)

技术

microbiological culture: suitable

颜色

beige

pH值（酸碱度）

6.8 (35.5 g/L in H2O)
6.8±0.2 (25 °C)

应用

food and beverages
microbiology

储存温度

15-25°C

适用性

Escherichia coli

相关类别

一般描述

GranuCult® prime Lauryl Sulfate broth (acc. ISO 4831, ISO 7251, and FDA-BAM) is used to selectively enrich and detect coliforms from food and animal feed, water, and other materials. The high nutrient quality and the presence of phosphate buffer ensure rapid growth and increased gas production of even the "slowly lactose-fermenting" coliform bacteria. Gas formation can be detected by using Durham tubes. The lauryl sulfate inhibits the growth of undesired bacteria.

应用

GranuCult® prime Lauryl Sulfate broth is suitable for the selective enrichment and presumptive detection of coliform bacteria from food and animal feed, water, and other materials.

特点和优势

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

联系

Replaces: 1.10266.5007

分析说明

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): >10⁴
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): >10⁴
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): none
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): none
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Incubation:
Escherichia coli: 24 ± 2 hours at 30 ± 1 °C and parallel 24 ± 2 hours at 37 ± 1 °C
Citrobacter: 24 ± 2 hours at 30 ± 1 °C
Enterococcus faecalis: 48 ± 2 hours at 30 ± 1 °C and parallel 48 ± 2 hours at 37 ± 1 °C

其他说明

  • The prepared medium is clear and yellowish-brown
  • Store the prepared medium in screw-capped containers at 2 °C to 8 °C in the dark and for up to one month

附注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

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