1.04030
VRB (Violet Red Bile Lactose)-MUG agar
FluoroCult®, FDA-BAM, suitable for microbiology, for Escherichia coli, for coliforms
别名:
Coliform selective agar, MUG Violet Red Bile Agar, VRB-MUG agar, Violet Red Bile Agar with MUG, Violet Red Bile Lactose-MUG agar
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About This Item
UNSPSC代码:
41106200
NACRES:
NA.74
推荐产品
Agency
FDA-BAM
质量水平
表单
medium granules (dehydrated (DCM))
包装
pkg of 500 g
制造商/商品名称
FluoroCult®
储存条件
(Keep tightly closed and dry.)
技术
microbiological culture: suitable
颜色
pink
pH值（酸碱度）
7.3-7.5 (25 °C, 39.6 g/L in H2O)
溶解性
39.6 g/L (after autoclaving)
堆积密度
620 kg/m3
应用
food and beverages
microbiology
储存温度
15-25°C
适用性
Escherichia coli
coliforms
一般描述
Fluorocult® VRB-MUG Agar is a selective medium for the detection and enumeration of coliform bacteria, in particular E. coli. Meat peptone provides amino acids and other complex nitrogenous substances, and yeast extract supplies Vitamin B complexes. Lactose is a fermentable carbohydrate. Crystal violet and bile salts inhibit the growth of accompanying Gram-positive bacterial flora. Neutral red acts as a pH indicator. Lactose-positive colonies show a color change to red due to acid production. Lactose-negative Enterobacteriaceae are colorless. Lactose-positive colonies are red and often surrounded by a turbid zone due to the precipitation of bile acids. E. coli colonies appear fluorescent under UV light. β-D-glucuronidase, which is produced by E. coli, cleaves 4-methylumbelliferyl-β-D-glucuronide (MUG) to 4-methylumbelliferone and glucuronide. The fluorogenic 4-methylumbelliferone can be detected under a long-wavelength UV lamp.
应用
Fluorocult® VRB Agar is suitable for the detection and enumeration of coliform bacteria, particularly E. coli, in food and dairy products.
特点和优势
- Fluorocult® fluorogenic culture media offer Rapid and Reliable culture testing
- Powerful alternatives to conventional microbiological methods for the rapid and sensitive detection of bacteria
- Offers superior granulated culture media
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
分析说明
Appearance (clearness): clear
Appearance (color): dark red
pH-value (25 °C): 7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): red with precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): red with precipitation halo
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): + (poor)
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): + (greenish)
Incubation: 18 - 24 hours at 35 °C aerobic.
Appearance (color): dark red
pH-value (25 °C): 7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): red with precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): red with precipitation halo
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): + (poor)
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): + (greenish)
Incubation: 18 - 24 hours at 35 °C aerobic.
法律信息
FLUOROCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 3
商品
Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
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