Anti-phospho-AMPK1 (Thr174) Antibody

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 174 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

This antibody recognizes phosphorylated AMPK1 at Thr174.

KLH-conjugated linear peptide corresponding to phosphorylated human AMPK1 (Thr174).

Anti-phospho-AMPK1 (Thr174) Antibody detects level of phospho-AMPK1 (Thr174) & has been published & validated for use in WB, IH.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot (SNAP ID) Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in HEK293 cell lysate (see page 2).

Evaluated by Western Blot in Lambda phosphatase treated and untreated HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate.

60 kDa

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

Maintain refrigerated at 2-8°C for up to 12 months.

Control
Lambda phosphatase treated and untreated Hek293 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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