Anti-phospho-AMPKα (Thr172) Antibody

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

This antibody recognizes AMPKα.

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat.

Anti-phospho-AMPKα (Thr172) Antibody is an antibody against phospho-AMPKα (Thr172) for use in WB.

Research Category
Signaling

Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot (SNAP ID) Analysis:

Evaluated by Western Blot in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected AMPKα in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

62 kDa

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Control
HEK293 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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