07-209-I
Anti-phospho-SHC Antibody (Tyr239, Tyr240)
from rabbit, purified by affinity chromatography
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p46Shc (pTyr194/pTyr195), p52Shc (pTyr239/pTyr240), p66Shc (pTyr349/pTyr350), SHC-transforming protein 1, tyrosine-phosphorylated, SH2 domain protein C1, tyrosine-phosphorylated, SHC-transforming protein 3, tyrosine-phosphorylated, SHC-transforming prote
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性(根据同源性预测)
mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology)
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
phosphorylation (pTyr239/pTyr240)
基因信息
human ... SHC1(6464)
一般描述
SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.
特异性
Numbering of the target pTyr residues varies depending on the species and spliced isoforms. Target pTyr residues this rabbit polyclonal detects correspond to pTyr349/pTyr350 of human spliced isoform 6 and p66Shc, pTyr239/pTyr240 of human spliced isoform 7 and p52Shc, pTyr194/pTyr195 of isoform p46Shc, and pTyr135/pTyr136 of isoform 5 reported by UniProt (P29353).
免疫原
KLH-conjugated linear peptide corresponding to a target site sequence surrounding phosphorylated Tyr239 and Tyr240 of human spliced isoform p52Shc.
应用
Detect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting.
质量
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells.
Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells.
目标描述
~52 kDa observed. 62.82/51.61/46.67/40.42/62.89/51.68 kDa (human isoform p66Shc/p52Shc/p46Shc/5/6/7), 62.61/51.41/46.47 kDa (mouse isoform p66Shc/p52Shc/p47Shc), and 51.50 kDa (rat) calculated.
其他说明
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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