Anti-DNMT3A2 Antibody

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation.

Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology

The antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples.

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2.

ELISA:
This antibody was shown by an outside laboratory to be suitable for ELISA.

Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha.

Western Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate.

~80 kDa

Protein A chromatography

Format: Purified

Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
L6, NIH/3T3, HeLa, or A431 cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.