05-623-AF488
Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor™ 488 Conjugate
clone CTD4H8, from mouse, ALEXA FLUOR™ 488
别名:
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
登录查看公司和协议定价
所有图片(2)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物来源
mouse
质量水平
偶联物
ALEXA FLUOR™ 488
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
CTD4H8, monoclonal
种属反应性
human, mouse
种属反应性（根据同源性预测）
yeast (based on 100% sequence homology), rat (based on 100% sequence homology)
技术
immunocytochemistry: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)
一般描述
DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
应用
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
质量
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.
目标描述
~210-220 kDa observed
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Lingtong Liu et al.
The Plant journal : for cell and molecular biology, 96(2), 421-437 (2018-07-27)
Sperm cell (SC) lineage development from the haploid microspore to SCs represents a unique biological process in which the microspore generates a larger vegetative cell (VC) and a smaller generative cell (GC) enclosed in the VC, then the GC further
Shao-Kuei Huang et al.
Current biology : CB, 31(22), 5102-5110 (2021-10-07)
The early Drosophila embryo provides unique experimental advantages for addressing fundamental questions of gene regulation at multiple levels of organization, from individual gene loci to the entire genome. Using 1.5-h-old Drosophila embryos undergoing the first wave of genome activation,1 we
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门