05-572
Anti-Caspase 9 Antibody, clone 96-2-22
clone 96-2-22, Upstate®, from mouse
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
96-2-22, monoclonal
Application:
WB
Citations:
27
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
96-2-22, monoclonal
Gene Information
human ... CASP9(842)
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
46/34 kDa
Immunogen
The N-terminal fragment of human Caspase 9 (amino acid residues 1-134)
Application
This Anti-Caspase 9 Antibody, clone 96-2-22 is validated for use in WB for the detection of Caspase 9.
Biochem/physiol Actions
Recognizes the proform and the active cleaved form of Caspase 9.
Physical form
Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431, Raji, or HFF cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-443
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lyndsay Davies et al.
Journal of cellular and molecular medicine, 13(8B), 1995-2007 (2008-12-02)
p53 apoptosis effector related to PMP-22 (PERP) is a transcriptional target gene of p53 tumour suppressor that is specifically induced during apoptosis and not during cell cycle arrest. In primary uveal melanoma (UM), the most common intraocular malignancy in adults
Ya-Fang Wang et al.
Journal of virology, 78(15), 7916-7924 (2004-07-16)
A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643 in mice. Strain MP4 exhibited a larger plaque size, grew more rapidly, and was more cytotoxic
Oncogene-dependent apoptosis is mediated by caspase-9.
Fearnhead, H O, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 13664-13669 (1998)
Morgan R Herod et al.
PloS one, 9(3), e90679-e90679 (2014-03-07)
Investigations into human norovirus infection, replication and pathogenesis, as well as the development of potential antiviral agents, have been restricted by the lack of a cell culture system for human norovirus. To date, the optimal cell culture surrogate virus model
Heat shock protein inhibitors increase the efficacy of measles virotherapy.
Liu, C; Erlichman, C; McDonald, CJ; Ingle, JN; Zollman, P; Iankov, I; Russell, SJ; Galanis, E
Gene Therapy null
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