05-253
Anti-ZAP-70 Antibody, clone 2F3.2
clone 2F3.2, Upstate®, from mouse
别名:
Anti-ADMIO2, Anti-SRK, Anti-STCD, Anti-STD, Anti-TZK, Anti-ZAP-70
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2F3.2, monoclonal
Application:
flow cytometry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
human
Citations:
53
Technique(s):
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-ZAP-70 Antibody, clone 2F3.2, clone 2F3.2, Upstate®, from mouse
isotype
IgG2a
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2F3.2, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ZAP70(7535)
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells
Application
For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.
Biochem/physiol Actions
Recognizes human ZAP-70, does not recognize murine ZAP-70.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
70 kDa
Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.
Immunogen
GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Genetic evidence of a role for Lck in T-cell receptor function independent or downstream of ZAP-70/Syk protein tyrosine kinases
Wong, J., et al
Molecular and cellular biology, 18, 2855-2866 (1998)
C Silvin et al.
The Journal of biological chemistry, 276(24), 21450-21457 (2001-04-03)
Wiskott-Aldrich syndrome protein (WASP) plays a key role in cytoskeletal rearrangement and transcriptional activation in T-cells. Recent evidence links WASP and related proteins to actin polymerization by the Arp2/3 complex. To study whether the role of WASP in actin polymerization
Eva Calpe et al.
PloS one, 8(12), e81221-e81221 (2013-12-07)
ZAP-70 in chronic lymphocytic leukemia (CLL) is associated with enhanced response to microenvironmental stimuli. We analyzed the functional consequences of ZAP-70 ectopic expression in malignant B-cells in a xenograft mouse model of disseminated B-cell leukemia. Mice injected with B-cells expressing
E M Schaeffer et al.
Science (New York, N.Y.), 284(5414), 638-641 (1999-04-24)
T cell receptor (TCR) signaling requires activation of Zap-70 and Src family tyrosine kinases, but requirements for other tyrosine kinases are less clear. Combined deletion in mice of two Tec kinases, Rlk and Itk, caused marked defects in TCR responses
Defective expression of the T-cell receptor-CD3 zeta chain in T-cell acute lymphoblastic leukaemia.
Giovanni F Torelli, Rossella Paolini, Caterina Tatarelli, Alessandra Soriani et al.
British Journal of Haematology null
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