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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
P2B11, monoclonal
种属反应性
mouse
种属反应性（根据同源性预测）
chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology)
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... MDC1(9656)
mouse ... Mdc1(240087)
一般描述
MDC1是粘连蛋白复合物的亚基，它是有丝分裂和减数分裂中姐妹染色单体粘连（sister chromatid cohesion）所需的关键蛋白。该蛋白的温度敏感性突变与姐妹染色单体粘连、染色体凝集和染色体分离的缺陷有关。 其表达受细胞周期调节，在S期达到最高。 MDC1的表达是响应DNA损伤而出现的S- 和 G2/M检查点介导的细胞周期阻滞所必需的。 研究显示MDC1可与DNA双链断裂位点附近的H2A.X 相互作用，并将ATM激酶募集至DNA的损伤区。与这种作用一致的是，MDC1 似乎调节其它涉及DNA损伤检查点的蛋白（如BRCA1和 Chk2）的功能。
特异性
该抗体识别N末端及周围的MDC1 。
免疫原
GST标记的重组蛋白，对应于N-末端及周围的小鼠MDC1。
应用
免疫细胞化学分析： 一个代表性批次的1：500稀释液在NIH/3T3细胞中检测到MDC1。
蛋白质印迹分析： 一个代表性批次已被独立实验室用于WB。(Lou, Z., et al. (2003).Nature.421(6926):957-961.)
蛋白质印迹分析： 一个代表性批次已被独立实验室用于WB。(Lou, Z., et al. (2003).Nature.421(6926):957-961.)
抗-MDC1抗体（克隆 P2B11）是用于检测 MDC1（也称为带BRCT结构域1的核因子）的小鼠单克隆抗体，&已在 ICC、& WB 中得到验证。
目标描述
~ 184 kDa 由于剪接作用人MDC1 有4种亚型 分子量大约为227、198、196和117 kDa
外形
形式：纯化
纯化的小鼠单克隆IgG1κ溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl，不含0.05％叠氮化钠的缓冲液中。
其他说明
浓度：请参考批次特异性浓缩物的检验报告。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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