选择尺寸
关于此项目
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human
General description
测定方案与现有的夹心ELISA方法相似,但有两个主要区别:
1)洗脱缓冲液破坏夹心结构,分离标记的检测抗体以进行定量。
2)Erenna 和SMCxPRO® 免疫测定系统使用单分子计数(SMC®)技术对分析物进行检测。
Application
Features and Benefits
- Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
- Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
- Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
- Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.
Packaging
Analysis Note
- Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 50-50,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 113% Plasma: 105%
- Recovery: Serum: 95% Plasma: 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Legal Information
Disclaimer
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
法规信息
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