ReadyTube^® Modified Semi-Solid RAPPAPORT-VASSILIADIS Medium

Peptone and casein hydrolysate provide nitrogenous, carbonaceous, B complex vitamins and other essential growth nutrients. Phosphate provides buffering capacity to the medium. Malachite green serves as a selective agent. The addition of novobiocin as a supplement in the medium selectively inhibits most Gram-positive organisms. Sodium chloride maintains the osmotic equilibrium of the media. Salmonella generally survives a slightly high osmotic pressure (due to MgCl₂ in the medium), grows at a slightly low pH, and is resistant to malachite green compared to other bacteria. Its efficiency is due to the ability of Salmonellae to migrate into the semi-solid medium, thus forming opaque halos of growth. All these factors make the medium selective for the isolation of Salmonella. Also, the semi-solid nature of the medium helps to differentiate the motile Salmonella from non-motile ones.

ReadyTube^® modified semi-Solid RAPPAPORT-VASSILIADIS medium is used for the selective enrichment and presumptive detection of Salmonella from food, animal feed, and environmental samples from primary production stage

ReadyTube^® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube^® modified semi-Solid RAPPAPORT-VASSILIADIS medium is used for the selective enrichment and presumptive detection of Salmonella sp. This medium is a semi-solid modification of Rappaport-Vassiliadis enrichment broth and is used for motility enrichment in Petri dishes. It is a rapid and sensitive medium for the isolation of Salmonella sp. and can be seeded directly after pre-enrichment.

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany