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796-0658

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

application for HPLC

Synonym(s):

Proteins, mixture

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About This Item

description

Separated on Discovery® BIO PolyMA-SCX

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
detector: UV, 280 nm

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

standard

Product No.
Description
Pricing

C4011

Cytochrome c from pigeon breast muscle, ≥95% based on Mol. Wt. 12,173 basis

C4879

α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder

C2037

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis

C3131

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

Cytochrome c from equine heart, suitable for GFC marker, BioReagent

C7752

Cytochrome c from equine heart, ≥95% based on Mol. Wt. 12,384 basis

C2506

Cytochrome c from equine heart, ≥95% (SDS-PAGE)

C2436

Cytochrome c from Saccharomyces cerevisiae, ≥85% based on Mol. Wt. 12,588 basis

R5125

Ribonuclease A from bovine pancreas, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5000

Ribonuclease A from bovine pancreas, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R6513

Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R5500

Ribonuclease A from bovine pancreas, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

R4875

Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R5250

Ribonuclease A from bovine pancreas, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5503

Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

C4129

α-Chymotrypsin from bovine pancreas, Type II, lyophilized powder, ≥40 units/mg protein

C3483

Cytochrome c from human heart, ≥95% (SDS-PAGE), lyophilized powder

30398

Cytochrome c from bovine heart, ≥95% (GE)

C2867

Cytochrome c from equine heart, BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

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