R6513
Ribonuclease A from bovine pancreas
for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Synonym(s):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
About This Item
Recommended Products
grade
for molecular biology
Quality Level
form
lyophilized
specific activity
≥70 Kunitz units/mg protein
mol wt
13.7 kDa
~13,700
foreign activity
Exonuclease and endonuclease, none detected
NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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General description
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
- for plasmid DNA purification
- to digest and avoid staining of double-stranded RNA during staining of nuclei
- in immunoperoxidase and immunofluorescence detection
- to treat cells prior to flow cytometry
- as a component of post-hybridization buffer for washing FFPE tissue sections post in-situ hybridization
- to treat cells during the preparation of outer membrane protein (OMP) extracts
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
Features and Benefits
Analysis Note
Other Notes
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Application
inhibitor
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the solubility of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
Stock solutions of RNase A can be made in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL.
What is the shelf life of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
This product is stable for at least 3 years when stored properly at -20 °C.
How are solutions of Product R6513, Ribonuclease A from bovine pancreas (RNase A), stored?
RNase A solutions can be stored, aliquoted and frozen at -20 °C.
Do I need to boil Product R6513, Ribonuclease A from bovine pancreas (RNase A), to inactivate residual DNases?
Boiling of stock solutions to inactivate residual DNase is not necessary and may cause precipitation of RNase with loss of enzymatic activity.
What working concentration of Product R6513, Ribonuclease A from bovine pancreas (RNase A), do you suggest?
We suggest using RNase A at a concentration of 10 ug/ml.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
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Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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