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R5503

Ribonuclease A from bovine pancreas

Type I-AS, 50-100 Kunitz units/mg protein

Synonym(s):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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About This Item

CAS Number:
9001-99-4
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
MFCD00132181
EC Number:
3.1.27.5 (BRENDA, IUBMB)

Key Documents

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Product Name

Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

biological source

bovine pancreas

type

Type I-AS

form

lyophilized powder

specific activity

50-100 Kunitz units/mg protein

mol wt

~13,700

technique(s)

cell based assay: suitable

impurities

salt, essentially free

suitability

suitable for mRNA or total RNA extracted from cells and tissues

application(s)

diagnostic assay manufacturing

foreign activity

protease, essentially free

storage temp.

−20°C

Quality Level

300

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Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Analysis Note

Protein determined by E.

Application

  • RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
  • RNase A is also used in RNA sequence analysis and protection assays.
  • RNase A has been used as a tool for computer-aided drug design.
  • RNase A supports the analysis of RNA sequences.
  • RNase A hydrolyze RNA contained in protein samples.
  • Purification of DNA is supported by RNase A.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Preparation Note

Salt fractionated and chromatographically purified.

wgk

WGK 3

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000491464
0000491464
0000482306
0000482307
0000482304

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P Klappa et al.
European journal of biochemistry, 254(1), 63-69 (1998-07-04)
Using a cross-linking approach, we have demonstrated that radiolabeled model peptides or misfolded proteins specifically interact in vitro with two different luminal proteins in a crude extract from sheep pancreas microsomes. One of the proteins was identified as protein disulphide-isomerase
J J Beintema et al.
Cellular and molecular life sciences : CMLS, 54(8), 825-832 (1998-10-07)
Enzymic properties of members of the ribonuclease A superfamily, like the activity on RNA, the preference for either cytosine or uracil in the primary binding site B1, the preference for the other side of the cleaved phosphodiester bond, the B2
M P Sasso et al.
Gene, 227(2), 205-212 (1999-02-19)
Molecular evolutionary analyses of mammalian ribonucleases have shown that gene duplication events giving three paralogous genes occurred in ruminant ancestors. The enzymes of the bovine species encoded by these genes, isolated from pancreas, brain and seminal vesicles, present similar enzymological
Gertrud Forika et al.
International journal of molecular sciences, 21(14) (2020-07-28)
The poor outcome of pancreas ductal adenocarcinomas (PDAC) is frequently linked to therapy resistance. Modulated electro-hyperthermia (mEHT) generated by 13.56 MHz capacitive radiofrequency can induce direct tumor damage and promote chemo- and radiotherapy. Here, we tested the effect of mEHT
Yanchang Li et al.
Molecular cell, 76(1), 126-137 (2019-08-25)
A surprising complexity of ubiquitin signaling has emerged with identification of different ubiquitin chain topologies. However, mechanisms of how the diverse ubiquitin codes control biological processes remain poorly understood. Here, we use quantitative whole-proteome mass spectrometry to identify yeast proteins
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Protocols

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

核糖核酸酶A(EC 3.1.27.5)的酶促测定

本实验方案可用于测定核糖核酸酶A(RNase A)的活性。

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