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Key Documents

796-0657

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

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500 G
¥749.51
1 KG
¥1,285.94

¥749.51

List Price¥1,199.21Save 37%

Available to ship on2025年4月25日Details

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500 G
¥749.51
1 KG
¥1,285.94

About This Item

¥749.51

List Price¥1,199.21Save 37%

Available to ship on2025年4月25日Details

description

Separated on Discovery® BIO PolyMA-WAX

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
detector: UV, 280 nm
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

suitability

application for HPLC

application(s)

life science and biopharma

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Ammonium bicarbonate puriss., meets analytical specification of Ph.Eur., BP, E 503, 99-101%

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Ammonium bicarbonate

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Ammonium bicarbonate

vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

product line

BioUltra

product line

ReagentPlus®

product line

-

product line

Vetec™

assay

≥99.5% (T)

assay

≥99.0%

assay

99-101%

assay

≥98.5%

form

powder or crystals

form

powder or crystals

form

powder or crystals

form

crystalline powder

impurities

insoluble matter, passes filter test

impurities

-

impurities

residual solvents, complies, tar like substances, complies, ≤0.001% heavy metals (as Pb), ≤0.01% non-volatile matter

impurities

-

pH

7.0-8.5 (25 °C, 1 M in H2O)

pH

7-8.5 (25 °C, 79.1 g/L)

pH

7-8.5 (25 °C, 79.1 g/L)

pH

7.0-8.5 (25 °C, 79.1 g/L)

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Trypsin Inhibitor

standard

Product No.
Description
Pricing

T1021

Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6

T9767

Trypsin inhibitor from Glycine max (soybean), BioReagent, For use as a marker in SDS-PAGE

T9003

Trypsin inhibitor from Glycine max (soybean), lyophilized powder

T9128

Trypsin inhibitor from Glycine max (soybean), lyophilized powder

T6522

Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture

M1882

Myoglobin from equine heart, ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

M9267

Myoglobin from equine heart, Isoelectric focusing marker, pI (1) 6.8, (2) 7.2

M6036

Myoglobin from human heart, ≥95% (SDS-PAGE), buffered aqueous glycerol solution

T0256

Trypsin inhibitor from bovine pancreas, Type I-P, essentially salt-free, lyophilized powder

C0880

Conalbumin from chicken egg white, Iron complex

C7786

Conalbumin from chicken egg white, BioReagent, suitable for cell culture

C0755

Conalbumin from chicken egg white, Substantially iron-free

T9378

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

T6414

Trypsin inhibitor from Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture

M0630

Myoglobin from equine skeletal muscle, 95-100%, essentially salt-free, lyophilized powder

T2011

Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)

T9253

Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor

93620

Trypsin inhibitor from Glycine max (soybean), lyophilized powder, ~10000 U/mg

M5696

Myoglobin from equine skeletal muscle, BioUltra, 95-100% (SDS-PAGE)

T2327

Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
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