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X4126

Sigma-Aldrich

Xylene Cyanol FF

BioReagent, Molecular Biology

Synonym(s):

Acid blue 147, Cyanol FF, XC

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10 G
CN¥382.62

CN¥382.62

Available to ship onApril 25, 2025Details

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10 G
CN¥382.62

About This Item

Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
Colour Index Number:
42135
EC Number:
220-167-5
MDL number:
MFCD00019481
UNSPSC Code:
12171500
PubChem Substance ID:
57654732
NACRES:
NA.52

CN¥382.62

Available to ship onApril 25, 2025Details

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

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1 of 4

This Item
335940B3269205486
Xylene Cyanol FF BioReagent, Molecular Biology

X4126

Xylene Cyanol FF

Xylene Cyanol FF Dye content ≥75 %

335940

Xylene Cyanol FF

Bromphenol Blue–Xylene Cyanole Dye solution lyophilized powder

B3269

Bromphenol Blue–Xylene Cyanole Dye solution

Xylenol Blue indicator grade, Dye content 90 %

205486

Xylenol Blue

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: suitable

suitability

suitable for nucleic acid staining

suitability

-

suitability

-

suitability

-

grade

for molecular biology

grade

-

grade

-

grade

indicator grade

solubility

H2O: complete 1 mg/mL, clear, blue

solubility

water: 1 mg/mL, blue

solubility

water: 0.2 vial/mL, blue

solubility

-

form

powder

form

powder or crystals

form

lyophilized powder

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
  • gel-loading dye[3]
  • formamide RNA loading dye[4]
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer[5]

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Certificates of Analysis (COA)

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    SHBS3724
    SHBR7866
    SHBM1857
    SHBL0446
    MKCF5562

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    Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
    <BIG>Ota M, et al.</BIG>
    Nature Protocols, 2857-2857 (2007)
    Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
    <BIG>Thiel WH, et al.</BIG>
    Methods in Molecular Biology, 187-199 (2015)
    Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
    <BIG>Afonin KA, et al.</BIG>
    Nature Protocols, 6, 2022-2022 (2011)
    Joseph Russo et al.
    Methods (San Diego, Calif.), 155, 116-123 (2018-12-07)
    Both RNA synthesis and decay must be balanced within a cell to achieve proper gene expression. Additionally, modulation of RNA decay specifically offers the cell an opportunity to rapidly reshape the transcriptome in response to specific stimuli or cues. Therefore
    André Vasconcelos et al.
    The breast journal, 8(4), 226-229 (2002-07-09)
    Breast cancer is the most frequent neoplasm in women. Expression of the estrogen receptor (ER) has a key role in breast cancer; the ER gene is located at chromosome 6q24-q27 and is made up of 8 exons with a total
    View All Related Papers

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