B3269
Bromphenol Blue–Xylene Cyanole Dye solution
lyophilized powder
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
Product Name
Bromphenol Blue–Xylene Cyanole Dye solution, lyophilized powder
form
lyophilized powder
color
dark blue
pH range
8-8.6
solubility
water: 0.2 vial/mL, blue
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Application
Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.
General description
Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.
Preparation Note
Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P2 (EN 143) respirator cartridges
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Genomic sequencing.
Church GM and Walter G
Proceedings of the National Academy of Sciences of the USA, 81.7, 1991-1995 (1984)
CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.
Wang Z, et al.
Methods, 48, 287-293 (2009)
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