B3269
Bromphenol Blue–Xylene Cyanole Dye solution
lyophilized powder
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UNSPSC Code:
12171500
NACRES:
NA.47
Recommended Products
form
lyophilized powder
Quality Level
color
dark blue
pH range
8-8.6
solubility
water: 0.2 vial/mL, blue
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Related Categories
General description
Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.
Application
Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.
Reconstitution
Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Genomic sequencing.
Church GM and Walter G
Proceedings of the National Academy of Sciences of the USA, 81.7, 1991-1995 (1984)
CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.
Wang Z, et al.
Methods, 48, 287-293 (2009)
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