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O3756

Sigma-Aldrich

Orange G

for NA electrophoresis

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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200 PKG
¥8,021.05

¥8,021.05

Estimated to ship on2025年5月06日Details

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200 PKG
¥8,021.05

About This Item

Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
1936-15-8
Molecular Weight:
452.37
Colour Index Number:
16230
Beilstein:
4120705
EC Number:
217-705-6
MDL number:
MFCD00012457
UNSPSC Code:
12171500
PubChem Substance ID:
57654483
NACRES:
NA.52

¥8,021.05

Estimated to ship on2025年5月06日Details

grade

Molecular Biology

Quality Level

200

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL, clear, orange to red

suitability

suitable for gel electrophoresis

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

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1 of 4

This Item
O725286128672176P
橙黄 G for NA electrophoresis

O3756

橙黄 G

橙黄 G certified by the BSC

O7252

橙黄 G

橙黄 G certified by the BSC, Dye content 80 %

861286

橙黄 G

橙黄 G certified by the BSC, suitable for histology

72176P

橙黄 G

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

for molecular biology

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

grade

-

suitability

suitable for gel electrophoresis

suitability

-

suitability

-

suitability

-

solubility

water: 1 mg/mL, clear, orange to red

solubility

water: 1 mg/mL, clear

solubility

H2O: 1 mg/mL

solubility

-

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

General description

Orange G is an azo dye[1] that is acidic in nature.[2] It is widely used as a tracking dye for nucleic acid gel electrophoresis to track DNA front in agarose gels. Typically runs at 50bp in up to 2.5% agarose.

Application

Orange G has been used as a DNA gel loading dye.[3][4][5]
Orange G is a tracking dye used in nucleic acid electrophoresis to track DNA front in agarose gels. Also used in SDS-capillary electrophoresis as a standard. Typically runs at 50bp in up to 2.5% agarose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBS3538
SHBR5176
SHBQ4761
SHBN4357
SHBN1695

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Degradation of azo dye Orange G in aqueous solutions by persulfate with ferrous ion
Xu XR and Li xZ
Separation and Purification Technology, 72(1), 105-111 (2010)
A Hiraoka et al.
Journal of chromatography. B, Biomedical sciences and applications, 697(1-2), 141-147 (1997-10-29)
Proteins of low molecular (M(r)) in human cerebrospinal fluid (CSF) were analyzed by capillary electrophoresis in a sodium dodecyl sulfate-containing polymer solution. Under the conditions employed, peaks of beta 2-microglobulin (beta MG) (M(r): 11,700), gamma-trace protein (12,300), myelin basic protein
Differential salt fractionation of nuclei to analyze chromatin-associated proteins from cultured mammalian cells
Herrmann C, et al.
Bio-protocol, 7(6), 1986-1986 (2017)
Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell
Nagano T, et al.
Nature Protocols, 10(12), 1986-1986 (2015)
Touchdown PCR for increased specificity and sensitivity in PCR amplification
Korbie DJ and Mattick JS
Nature Protocols, 3(9), 1452-1452 (2008)
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