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Merck
CN

B8026

Bromophenol Blue sodium salt

For molecular biology, powder

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
NACRES:
NA.47
PubChem Substance ID:
24892042
UNSPSC Code:
12171500
EC Number:
252-170-2
MDL number:
MFCD00013793
Beilstein/REAXYS Number:
6995701

Key Documents

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Product Name

Bromophenol Blue sodium salt, Molecular Biology, suitable for electrophoresis

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

grade

Molecular Biology

form

powder

technique(s)

electrophoresis: suitable

visual transition interval

3.0-4.6, yellow to blue

ε (extinction coefficient)

≥150000 at 597-603 nm in H2O
≥17000 at 250-256 nm in H2O
≥23000 at 306-312 nm in H2O

suitability

suitable for (Suitable For Use as a Tracking Dye For Electrophoresis in Alkaline and Neutral Buffers)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Bromophenol Blue sodium salt has been used in the buffer solution to differentiate dead and living cells. It has also been used as a constituent in gel loading buffer.

General description

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000463672
0000463672
SHBS8422
0000329856
0000231405

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